Cleft Palate Formation in FetalBrMice with Midfacial Retrusion: Tenascin, Fibronectin, Laminin, and Type IV Collagen Immunolocalization

1998 ◽  
Vol 35 (1) ◽  
pp. 65-76 ◽  
Author(s):  
G. D. Singh ◽  
J. Johnston ◽  
W. Ma ◽  
S. Lozanoff
Keyword(s):  
Cleft Palate ◽  
Type Iv Collagen ◽  
Type Iv

Cleft Palate Formation in Fetal Br Mice with Midfacial Retrusion: Tenascin, Fibronectin, Laminin, and Type IV Collagen Immunolocalization

1998 ◽  
Vol 35 (1) ◽  
pp. 65-76 ◽  
Author(s):  
G.D. Singh ◽  
J. Johnston ◽  
W. Ma ◽  
S. Lozanoff
Keyword(s):  
Extracellular Matrix ◽  
Cleft Palate ◽  
Type Iv Collagen ◽  
Craniofacial Morphology ◽  
Homogeneous Distribution ◽  
Secondary Antibody ◽  
Type Iv ◽  
Rabbit Igg ◽  
Secondary Palate ◽  
Immunohistological Analysis

Objective This study tested the hypothesis that altered craniofacial morphology does not affect the expression of extracellular matrix (ECM) molecules such as fibronectin (FN), laminin (LN), type IV collagen, and tenascin-C (TN) but is associated with failure of palatal shelf elevation and fusion concomitant with cleft palate formation. Design To test this hypothesis, a comparative immunohistological analysis of FN, LN, type IV collagen, and TN was undertaken on brachyrrhine (Br/Br) mice and normal (+/+) fetuses during secondary palate formation. Normal and Br/Br fetuses were collected at gestational days E13 and E14 (representing prefusion stages) and E15 and E18 (representing postfusion stages). Cryostat palatal sections (8 μm) were postfixed in methanol, washed, and stained with primary antibody. All sections were washed and coated with secondary antibody (swine-anti-rabbit IgG) and mounted with citifluor. Results Immunohistological analysis showed that LN and type IV collagen were located near the presumptive medial epithelial seam (MES) or edge (MEE) in +/+ or Br/Br fetuses, respectively. Fibronectin showed a homogeneous distribution at all stages in both groups of mice. In contrast, TN became localized below the presumptive MES or MEE in both groups of mice at E14. In +/+ animals at E15, TN dissipated and became confined to the oral basement membrane by E18. At E15 and E18 in cleft Br/Br mutants, TN stained beneath the MEE. Conclusion Although the distributions of ECM molecules are similar during normal and cleft palatogenesis, differences in TN expression are associated with cleft palate formation.

The use of 1nm silver enhanced gold probes for the detection of skin antigens

1990 ◽  
Vol 48 (3) ◽  
pp. 902-903
Author(s):  
J.P Cassella ◽  
H. Shimizu ◽  
A. Ishida-Yamamoto ◽  
R.A.J. Eady
Keyword(s):  
Type Iv Collagen ◽  
Freeze Substitution ◽  
Collagen Type Iv ◽  
Electron Microscopic ◽  
Normal Human Skin ◽  
Type Iv ◽  
Type Vii Collagen ◽  
Keratin Filaments ◽  
Normal Human ◽  
Phosphate Buffered Saline

1nm colloidal gold with silver enhancement has been used in conjunction with a low-temperature post-embedding (post-E) technique for the demonstration of skin antigens at both the light microscopic (LM) and electron microscopic (EM) levels.Keratin filaments and basement membrane zone (BMZ) associated antigens in normal human skin (NHS) were immunolabelled using antibodies against keratin 14, 10, and 1, the carboxy-terminus and collagenous portion of type VII collagen, type IV collagen and bullous pemphigoid antigen (BP-Ag).Fresh samples of NHS were cryoprotected in 15% glycerol, cryofixed in propane at -190°C, subjected to freeze substitution in methanol at -80°C and embedded in Lowicryl K11M at -60°C. Polymerisation of the resin was initiated under UVR at - 60°C for 48 hours and continued at room temperature for a further 48 hours. Semith in sections were air dried onto slides coated with 3-aminopropyltriethoxysilane. The following immunolabelling protocol was adopted: Primary antibody was applied for 2 hours at 37°C or overnight at 4°C. Following washing in Dulbecco’s phosphate buffered saline (PBSA) a biotinylated secondary antibody was applied for 2 hours at 37°C. The sections were further washed in PBSA and 1nm gold avidin was applied. Sections were finally washed in PBSA and silver enhanced.

scholarly journals Complete primary structure of a sea urchin type IV collagen alpha chain and analysis of the 5' end of its gene.

1993 ◽  
Vol 268 (7) ◽  
pp. 5249-5254
Author(s):  
J.Y. Exposito ◽  
M. D'Alessio ◽  
M. Di Liberto ◽  
F. Ramirez
Keyword(s):  
Sea Urchin ◽  
Primary Structure ◽  
Type Iv Collagen ◽  
Alpha Chain ◽  
Type Iv ◽  
Its Gene ◽  
Complete Primary Structure

scholarly journals Dynamically remodeled hepatic extracellular matrix predicts prognosis of early-stage cirrhosis

2021 ◽  
Vol 12 (2) ◽  
Author(s):  
Yuexin Wu ◽  
Yuyan Cao ◽  
Keren Xu ◽  
Yue Zhu ◽  
Yuemei Qiao ◽  
...  
Keyword(s):  
Liver Cirrhosis ◽  
Basement Membrane ◽  
Type Iv Collagen ◽  
Patient Survival ◽  
Early Stage ◽  
Ecm Remodeling ◽  
Type Iv ◽  
Ecm Proteins ◽  
Stage Disease ◽  
Cirrhotic Patients

AbstractLiver cirrhosis remains major health problem. Despite the progress in diagnosis of asymptomatic early-stage cirrhosis, prognostic biomarkers are needed to identify cirrhotic patients at high risk developing advanced stage disease. Liver cirrhosis is the result of deregulated wound healing and is featured by aberrant extracellular matrix (ECM) remodeling. However, it is not comprehensively understood how ECM is dynamically remodeled in the progressive development of liver cirrhosis. It is yet unknown whether ECM signature is of predictive value in determining prognosis of early-stage liver cirrhosis. In this study, we systematically analyzed proteomics of decellularized hepatic matrix and identified four unique clusters of ECM proteins at tissue damage/inflammation, transitional ECM remodeling or fibrogenesis stage in carbon tetrachloride-induced liver fibrosis. In particular, basement membrane (BM) was heavily deposited at the fibrogenesis stage. BM component minor type IV collagen α5 chain expression was increased in activated hepatic stellate cells. Knockout of minor type IV collagen α5 chain ameliorated liver fibrosis by hampering hepatic stellate cell activation and promoting hepatocyte proliferation. ECM signatures were differentially enriched in the biopsies of good and poor prognosis early-stage liver cirrhosis patients. Clusters of ECM proteins responsible for homeostatic remodeling and tissue fibrogenesis, as well as basement membrane signature were significantly associated with disease progression and patient survival. In particular, a 14-gene signature consisting of basement membrane proteins is potent in predicting disease progression and patient survival. Thus, the ECM signatures are potential prognostic biomarkers to identify cirrhotic patients at high risk developing advanced stage disease.

scholarly journals Physical and immunochemical studies of the globular domain of type IV collagen. Cryptic properties of the Goodpasture antigen.

1985 ◽  
Vol 260 (14) ◽  
pp. 8564-8570 ◽  
Author(s):  
J Wieslander ◽  
J Langeveld ◽  
R Butkowski ◽  
M Jodlowski ◽  
M Noelken ◽  
...  
Keyword(s):  
Type Iv Collagen ◽  
Globular Domain ◽  
Type Iv ◽  
Goodpasture Antigen

scholarly journals Cell surface-associated proteins which bind native type IV collagen or gelatin.

1984 ◽  
Vol 259 (9) ◽  
pp. 5915-5922 ◽  
Author(s):  
M Kurkinen ◽  
A Taylor ◽  
J I Garrels ◽  
B L Hogan
Keyword(s):  
Cell Surface ◽  
Type Iv Collagen ◽  
Type Iv ◽  
Associated Proteins
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